ARL2 antibody (C-Term)

Catalog No. ABIN184799

Product Details anti-ARL2 Antibody

Target: ARL2 (ADP-Ribosylation Factor-Like 2 (ARL2))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: ARL2

Sequence: LDDISSRIFT AD

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-LDDISSRIFTAD, from the C Terminus of the protein sequence according to NP_001658.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 18 kDa band observed in lysates of cell line A431 and approx. 20 kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9 kDa according to NP_001658.2). Recommended concentration: 0.5-1 μg/mL.Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARL2

Target: ARL2 (ADP-Ribosylation Factor-Like 2 (ARL2))

Alternative Name: ARL2 (ARL2 Products)

Synonyms: arfl2 antibody, MGC75676 antibody, ARFL2 antibody, 2610009M23Rik antibody, AI115441 antibody, AW553335 antibody, ADP ribosylation factor like GTPase 2 antibody, ADP-ribosylation factor-like 2 antibody, ADP-ribosylation factor like GTPase 2 antibody, arl2 antibody, ARL2 antibody, Arl2 antibody

Background: ARL2, ADP-ribosylation factor-like 2, ARFL2, ADP=ribosylation factor-like 2

Gene ID: 402

NCBI Accession: NP_001658

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location