PAX8 antibody (N-Term)
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- Target See all PAX8 Antibodies
- PAX8 (Paired Box 8 (PAX8))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PAX8 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- PAX8 (N Terminus)
- Sequence
- PHNSIRSGHG GLN
- Specificity
- This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1.
- Isotype
- IgG
- Top Product
- Discover our top product PAX8 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Kidney Cortex. Recommended concentration: 5 μg/mL.
Western Blot: Approx 35 kDa band observed in Human Kidney lysates. An additional 48 kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9 kDa according to Human NP_039247.1 PAX8D and 48.2 kDa according to Human NP_003457.1 PAXA).
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PAX8 (Paired Box 8 (PAX8))
- Alternative Name
- PAX8 (PAX8 Products)
- Synonyms
- PAX8 antibody, XPax-8 antibody, XPax8 antibody, pax-8 antibody, PAX-8 antibody, Pax-8 antibody, paired box 8 antibody, paired box 8 L homeolog antibody, PAX8 antibody, pax8 antibody, Pax8 antibody, pax8.L antibody
- Background
- PAX8, paired box gene 8, paired domain gene 8, paired box 8
- Gene ID
- 7849, 18510, 81819
- NCBI Accession
- NP_003457, NP_039245, NP_039246, NP_039247, NP_054698
- Pathways
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation
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