KIF5B antibody (C-Term)

Catalog No. ABIN184774

Product Details anti-KIF5B Antibody

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KIF5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: Kinesin 1 / UKHC

Sequence: QPVAVRGGGG KQV

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Immunofluorescence:This antibody has been successfully used in IF on Human: Connell et al. (20

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-KIF5B antibody (ABIN184774)

Connell, Allison, Rodger, Pearson, Zlamalova, Reid: "ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic." in: Cellular and molecular life sciences : CMLS, Vol. 77, Issue 13, pp. 2641-2658, (2020) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Yasuda, Clatterbuck-Soper, Jackrel, Shorter, Mili: "FUS inclusions disrupt RNA localization by sequestering kinesin-1 and inhibiting microtubule detyrosination." in: The Journal of cell biology, Vol. 216, Issue 4, pp. 1015-1034, (2017) (PubMed).

Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, (2010) (PubMed).

Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, (2008) (PubMed).

Target Details for KIF5B

Target: KIF5B (Kinesin Family Member 5B (KIF5B))

Alternative Name: KIF5B (KIF5B Products)

Synonyms: KINH antibody, KNS antibody, KNS1 antibody, UKHC antibody, Khc antibody, AL022807 antibody, Khcs antibody, Kns1 antibody, Ukhc antibody, khc antibody, KIF5A antibody, kinesin family member 5B antibody, kinesin heavy chain antibody, kinesin-1 antibody, kinesin-1 heavy chain antibody, kinesin family member 5B S homeolog antibody, KIF5B antibody, LOC411769 antibody, cgd6_2790 antibody, kin-1 antibody, Tb927.1.1350 antibody, LACBIDRAFT_305999 antibody, Khc antibody, LOC100538629 antibody, Kif5b antibody, kif5b.S antibody

Background: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain

Gene ID: 3799

NCBI Accession: NP_004512

Pathways: Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization