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Iba1 antibody (C-Term)

Verified IBA1 Reactivity: Human, Rat, Mouse WB, IHC, ELISA Host: Goat Polyclonal unconjugated

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human Frontal Cortex (A), Rat Brain (B), Mouse (C) Brain and Pig Brain (D) lysates (35µg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 (5µg/ml) staining of paraffin embedded Human Lung. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human Brain (Frontal Cortex (A) Rat (B) Mouse (C) Brain and Swine E) Brain lysates (35µg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human (A) and (0. (Iba1 antibody (C-Term))

ABIN184738 (2µg/ml) staining of paraffin embedded Mouse Brain. (Iba1 antibody (C-Term))

ABIN184738 (0. (Iba1 antibody (C-Term))

AIF1 / IBA1 staining of Rat Brain lysate (35 µg protein in RIPA buffer) by ABIN184738 (0. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human (A) and (0. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human Frontal Cortex (A) and (0. (Iba1 antibody (C-Term))

ABIN184738 Negative Control showing staining of paraffin embedded Mouse Lung, with no primary antibody. (Iba1 antibody (C-Term))

ABIN184738 Staining of paraffin embedded Mouse Lung. (Iba1 antibody (C-Term))

ABIN184738 (1µg/ml) staining of Human Lymph node (A), Mouse Brain (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 (1 μg/mL) staining of Mouse (A) and (2 μg/mL) Rat (B Brain lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

ABIN184738 (6 μg/mL) staining of paraffin embedded Human Placenta. (Iba1 antibody (C-Term))

ABIN184738 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (1 μg/mL) staining of Mouse (A) and Rat (B) Brain lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

(ABIN184738) (5 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

B05419 (0. (Iba1 antibody (C-Term))

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (0. (Iba1 antibody (C-Term))

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Lung. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (2 μg/mL) staining of Rat (A) and Mouse (B) Brain lysates (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

(ABIN184738) (4 μg/mL) staining of paraffin embedded Human Placenta. (Iba1 antibody (C-Term))

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysates (35 μg protein in RIPA buffer) Detected by chemiluminescence. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

B05419 (0. (Iba1 antibody (C-Term))

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex (A) Mouse Brain (B) and Rat Brain (C) lysate (35 μg protein in RIPA buffer). (Iba1 antibody (C-Term))

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody. (Iba1 antibody (C-Term))

(ABIN184738) (0. (Iba1 antibody (C-Term))

(ABIN184738) (4 μg/mL) staining of paraffin embedded Human Spleen. (Iba1 antibody (C-Term))

41 images

Catalog No. ABIN184738

Datasheet as PDF

Target See all Iba1 (IBA1) Antibodies

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
Binding Specificity
16

16

15

9

8

8

7

6

3

1

1

1

1

1

1

1
C-Term
Reactivity
94

57

52

1

1
Human, Rat, Mouse
Host
83

32

4
Goat
Compatible Secondaries
Clonality
86

33
Polyclonal
Conjugate
49

17

13

6

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
This Iba1 antibody is un-conjugated
Application
71

43

38

22

17

14

14

11

11

6

5

4

3

3

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose

AIF1/IBA1 isoform 1 and 3

Sequence

TGPPAKKAIS ELP

Specificity

This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Cross-Reactivity

Human, Mouse, Pig, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3.

Isotype

IgG

Top Product

Discover our top product IBA1 Primary Antibody
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
IBA1 Reactivity: Human WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody
IBA1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 Reactivity: Human WB, IHC, IP, ICC, IF, IHC (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C5 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibody
Verified IBA1 Reactivity: Human IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibody
IBA1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-146) antibody
IBA1 Reactivity: Human IHC, StM Host: Mouse Monoclonal AIF1-1909 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 6-36), (N-Term) antibody
IBA1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB14652 unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx. 16-17 kDa band observed in Human Frontal Cortex and in Mouse and Rat Brain lysates (calculated MW of 16.7 kDa according to Human NP_001614.3, 16.9 kDa according to Mouse NP_062340.1 and 16.8 kDa according to Rat NP_058892.1). Recommende
Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name

AIF1 (IBA1 Products)

Synonyms

AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody

Background

AIF1, IBLY409840A1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8

Gene ID

199, 11629, 29427

NCBI Accession

NP_116573, NP_001614

Pathways

Smooth Muscle Cell Migration

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