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APEX1 antibody (N-Term)

Verified APEX1 Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN184704
  • Target See all APEX1 Antibodies
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    Binding Specificity
    • 7
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 89
    • 43
    • 39
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 74
    • 17
    • 1
    Goat
    Clonality
    • 75
    • 17
    Polyclonal
    Conjugate
    • 63
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This APEX1 antibody is un-conjugated
    Application
    • 79
    • 29
    • 28
    • 23
    • 18
    • 11
    • 7
    • 6
    • 6
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    APE1 / APEX1
    Sequence
    PKRGKKGAVA EDGD
    Specificity
    Reported variants represent identical protein (NP_001632.2, NP_542379.1, NP_542380.1).
    Cross-Reactivity
    Cow, Dog, Human, Pig
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2.
    Isotype
    IgG
    Top Product
    Discover our top product APEX1 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6 μg/mL.

    Western Blot: Approx 37 kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6 kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    Alternative Name
    APEX1 (APEX1 Products)
    Background
    APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, a
    Gene ID
    328
    NCBI Accession
    NP_001632
    Pathways
    DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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