RACGAP1 antibody (C-Term)

Catalog No. ABIN184690

Product Details anti-RACGAP1 Antibody

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RACGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: RACGAP1 / MgcRacGAP

Sequence: GRQGNFFASP MLK

Specificity: This antibody is expected to recognize all reported isoforms (NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_0011

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0 kDa according to NP_037409.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room te

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RACGAP1

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Alternative Name: RACGAP1 (RACGAP1 Products)

Synonyms: CYK4 antibody, HsCYK-4 antibody, ID-GAP antibody, MgcRacGAP antibody, cyk-4 antibody, id-gap antibody, mgcracgap antibody, RACGAP1 antibody, MGC121578 antibody, Gap antibody, RasGAP antibody, Rasa antibody, GAPX antibody, AI227039 antibody, AI327394 antibody, Band25 antibody, GTPase antibody, gtl11 antibody, mKIAA1478 antibody, wu:fb92c08 antibody, zgc:77839 antibody, Rac GTPase activating protein 1 antibody, rac GTPase-activating protein 1 antibody, Rac GTPase activating protein 1 L homeolog antibody, RAS p21 protein activator 1 antibody, Rac GTPase-activating protein 1 antibody, si:ch1073-416j23.1 antibody, RACGAP1 antibody, racgap1 antibody, racgap1.L antibody, racgap1.2.L antibody, racgap1.2 antibody, Rasa1 antibody, Racgap1 antibody, si:ch1073-416j23.1 antibody

Background: RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4

Gene ID: 29127

NCBI Accession: NP_037409, NP_001306934, NP_001306935, NP_001306936

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor