Annexin A2 antibody (N-Term)

Catalog No. ABIN184687

Product Details anti-Annexin A2 Antibody

Target: Annexin A2 (ANXA2)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Annexin A2

Sequence: STVHEILCKL SLEGD

Specificity: This antibody is expected to recognise both reported isoforms (NP_001002858.1, NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence STVHEILCKLSLEGD, from the N Terminus of the protein sequence according to NP_001002858.1, NP_004030.1, NP_001002857.1, NP_001129487.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in lysates of cell lines A549, HeLa and NIH3T3 (calculated MW of 38.6 kDa according to NP_004030.1 and NP_001002858.1). Recommended concentration: 0.5-3 μg/mL. Primary incubation was for 1 hour.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: ANXA2 (ANXA2 Products)

Background: ANXA2, Annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, HEL-S-270, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypep

Gene ID: 302, 12306, 56611

NCBI Accession: NP_001002858, NP_004030

Pathways: S100 Proteins