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SH3GLB1 antibody (C-Term)

Verified SH3GLB1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN184621
  • Target See all SH3GLB1 Antibodies
    SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))
    Binding Specificity
    • 15
    • 8
    • 8
    • 7
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 61
    • 10
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 57
    • 4
    • 1
    Goat
    Clonality
    • 58
    • 4
    Polyclonal
    Conjugate
    • 28
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SH3GLB1 antibody is un-conjugated
    Application
    • 43
    • 26
    • 14
    • 13
    • 13
    • 9
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purpose
    BIF-1 / SH3GLB1
    Sequence
    QKGKVPITYL ELLN
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 40 kDa band observed in lysates of cell lines HEK293 and NIH3T3, and approx. 38 kDa in lysates of cell lines A431, RAW264.7 and KNRK (calculated MW of 40.8 kDa according to Human NP_057093.1, Mouse NP_062337.1 and Rat NP_001011929.1).

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))
    Alternative Name
    SH3GLB1 (SH3GLB1 Products)
    Background
    SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1, CGI-61, KIAA0491, endophilin B1, Bax-interacting factor 1, SH3-containing protein SH3GLB1, SH3-domain, GRB2-like, endophilin B1, SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1, dJ612B15.2
    Gene ID
    51100, 54673
    NCBI Accession
    NP_057093
    Pathways
    Autophagy
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