SH3GLB1 antibody (C-Term)

Catalog No. ABIN184621

Product Details anti-SH3GLB1 Antibody

Target: SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SH3GLB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: BIF-1 / SH3GLB1

Sequence: QKGKVPITYL ELLN

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QKGKVPITYLELLN, from the C Terminus of the protein sequence according to NP_057093.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell lines HEK293 and NIH3T3, and approx. 38 kDa in lysates of cell lines A431, RAW264.7 and KNRK (calculated MW of 40.8 kDa according to Human NP_057093.1, Mouse NP_062337.1 and Rat NP_001011929.1).

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SH3GLB1

Target: SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))

Alternative Name: SH3GLB1 (SH3GLB1 Products)

Background: SH3GLB1, Bif-1, SH3-domain GRB2-like endophilin B1, CGI-61, KIAA0491, endophilin B1, Bax-interacting factor 1, SH3-containing protein SH3GLB1, SH3-domain, GRB2-like, endophilin B1, SH3-containing protein SH3GLB1 CGI-61 protein endophilin B1, dJ612B15.2

Gene ID: 51100, 54673

NCBI Accession: NP_057093

Pathways: Autophagy