STAP2 antibody (C-Term)

Catalog No. ABIN184610

Product Details anti-STAP2 Antibody

Target: STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This STAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: STAP2 / BKS

Sequence: ELQKKLEKRR ALEH

Specificity: This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ELQKKLEKRRALEH, from the C Terminus of the protein sequence according to NP_060190.2, NP_001013863.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Human Heart lysates and 45 kDa band observed in Human Breast Lysates (calculated MW of 49.6 kDa according to NP_060190 and of NP_001013863.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-STAP2 antibody (ABIN184610)

Ikeda, Mizushima, Sekine, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells." in: Cancer science, Vol. 102, Issue 4, pp. 756-61, (2011) (PubMed).

Ikeda, Sekine, Mizushima, Nakasuji, Miyasaka, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells." in: The Journal of biological chemistry, Vol. 285, Issue 49, pp. 38093-103, (2010) (PubMed).

Ikeda, Miyasaka, Sekine, Mizushima, Muromoto, Nanbo, Yoshimura, Matsuda: "STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation." in: Biochemical and biophysical research communications, Vol. 384, Issue 1, pp. 71-5, (2009) (PubMed).

Sekine, Ikeda, Tsuji, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7966-74, (2009) (PubMed).

Target Details for STAP2

Target: STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

Alternative Name: STAP2 (STAP2 Products)

Synonyms: BKS antibody, AW049765 antibody, STAP-2 antibody, wu:fa55b07 antibody, zgc:77021 antibody, cb901 antibody, stap2 antibody, wu:fd15d07 antibody, zgc:63913 antibody, signal transducing adaptor family member 2 antibody, signal transducing adaptor family member 2b antibody, signal transducing adaptor family member 2a antibody, STAP2 antibody, Stap2 antibody, stap2b antibody, stap2a antibody

Background: STAP2, BKS, STAP-2, FLJ20234, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein-2, signal transducing adaptor family member 2

Gene ID: 55620

NCBI Accession: NP_060190, NP_001013863