MAX antibody (C-Term)

Catalog No. ABIN184586

Product Details anti-MAX Antibody

Target: MAX (MYC Associated Factor X (MAX))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MAX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: MAX

Sequence: EEPQSRKKLR MEAS

Specificity: This antibody is expected to recognize both reported isoforms (NP_002373.3, NP_660087.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EEPQSRKKLRMEAS, from the C Terminus of the protein sequence according to NP_002373.3, NP_660087.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.

Western Blot: Approx 22 kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3 kDa according to NP_002373.3). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10μg/ml.

Additional validation: This antibody has been successfully used in the followin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAX

Target: MAX (MYC Associated Factor X (MAX))

Alternative Name: MAX (MAX Products)

Synonyms: CG9648 antibody, Dmel\\CG9648 antibody, MAX antibody, bHLHd9 antibody, dMax antibody, dmax antibody, max antibody, bhlhd4 antibody, bhlhd5 antibody, bhlhd6 antibody, bhlhd7 antibody, bhlhd8 antibody, xmax2 antibody, xmax4 antibody, xmax antibody, AA960152 antibody, AI875693 antibody, bHLHd4 antibody, bHLHd5 antibody, bHLHd6 antibody, bHLHd7 antibody, bHLHd8 antibody, fa92b04 antibody, fb52a04 antibody, fb95h11 antibody, wu:fa92b04 antibody, wu:fb52a04 antibody, wu:fb95h11 antibody, zmax antibody, CG9648 gene product from transcript CG9648-RD antibody, MYC associated factor X L homeolog antibody, MYC associated factor X antibody, Max protein antibody, MYC associated factor X S homeolog antibody, myc associated factor X antibody, Max antibody, max.L antibody, MAX antibody, max antibody, max.S antibody

Background: MAX, MAX protein, MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, myc-associated factor X, helix-loop-helix zipper protein, MYC associated factor X, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1

Gene ID: 4149, 17187

NCBI Accession: NP_002373, NP_660087

Pathways: Mitotic G1-G1/S Phases