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NCF1 antibody (C-Term)

Verified NCF1 Reactivity: Human, Mouse, Pig WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN184583
  • Target See all NCF1 Antibodies
    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
    Binding Specificity
    • 28
    • 16
    • 12
    • 11
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 127
    • 48
    • 44
    • 16
    • 8
    • 3
    • 3
    • 3
    • 3
    • 3
    Human, Mouse, Pig
    Host
    • 140
    • 1
    • 1
    Goat
    Clonality
    • 140
    • 1
    Polyclonal
    Conjugate
    • 72
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This NCF1 antibody is un-conjugated
    Application
    • 103
    • 65
    • 60
    • 26
    • 23
    • 17
    • 14
    • 13
    • 8
    • 8
    • 4
    • 4
    • 3
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    NCF1 / p47phox
    Sequence
    SESTKRKLAS AV
    Cross-Reactivity
    Cow, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45

    Peptide ELISA: antibody detection limit dilution 1:20000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
    Alternative Name
    NCF1 (NCF1 Products)
    Synonyms
    NCF1A antibody, NOXO2 antibody, SH3PXD1A antibody, p47phox antibody, Ncf-1 antibody, p47 antibody, neutrophil cytosolic factor 1 antibody, NCF1 antibody, Ncf1 antibody
    Background
    NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2
    Gene ID
    653361, 17969, 114553
    NCBI Accession
    NP_000256
    Pathways
    PI3K-Akt Signaling
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