Aurora A antibody (C-Term)
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- Target See all Aurora A (AURKA) Antibodies
- Aurora A (AURKA) (Aurora Kinase A (AURKA))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Aurora A antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Purpose
- Aurora kinase A / AURKA
- Sequence
- QNKESASKQS
- Specificity
- Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QNKESASKQS, from the C Terminus of the protein sequence according to NP_003591.2.
- Isotype
- IgG
- Top Product
- Discover our top product AURKA Primary Antibody
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- Application Notes
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Western Blot: Approx 45 kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8 kDa according to NP_003591.2). Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from a customer).
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Aurora A (AURKA) (Aurora Kinase A (AURKA))
- Alternative Name
- AURKA (AURKA Products)
- Synonyms
- AIK antibody, ARK1 antibody, AURA antibody, AURORA2 antibody, BTAK antibody, PPP1R47 antibody, STK15 antibody, STK6 antibody, STK7 antibody, AIRK1 antibody, AU019385 antibody, AW539821 antibody, Ark1 antibody, Aurora-A antibody, Ayk1 antibody, IAK antibody, IAK1 antibody, Stk6 antibody, 87A7-9/2 antibody, Aur-A antibody, AurA antibody, CG3068 antibody, Dmel\\CG3068 antibody, aur-A antibody, aurA antibody, ck10 antibody, ck[10] antibody, l(3)87Ac antibody, l(3)ck10 antibody, ARK-1 antibody, Eg2 antibody, aik antibody, ark1 antibody, aur-a antibody, aura antibody, aurka-a antibody, aurora2 antibody, btak antibody, stk15 antibody, stk6 antibody, stk7 antibody, pEg2 antibody, wu:fb37c10 antibody, wu:fc02c02 antibody, zgc:100912 antibody, AURKA antibody, aurka antibody, aurora kinase A antibody, aurora A antibody, aurora kinase A L homeolog antibody, hypothetical protein antibody, aurora kinase A S homeolog antibody, AURKA antibody, Aurka antibody, aurA antibody, aurka.L antibody, aurka antibody, Aurora-A antibody, aurka.S antibody
- Background
- AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, ser
- Gene ID
- 6790
- NCBI Accession
- NP_003591
- Pathways
- Cell Division Cycle, Asymmetric Protein Localization
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