tau antibody (Ser396)

Catalog No. ABIN1842565

Product Details anti-tau Antibody

Target: tau (tau Protein)

Binding Specificity: Ser396

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-Tau (Ser396) Polyclonal Antibody detects endogenous levels of human, mouse, and rat tau protein.

Characteristics: Rabbit Anti-Tau (Ser396) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to residues surrounding serine 396 of human tau.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide corresponding to residues surrounding Ser396 of human tau

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 5-1 µg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

References for anti-tau antibody (ABIN1842565)

Abbasian, Burton, Herbert, Tregunna, Brown, Ghaderi-Najafabadi, Brunskill, Goodall, Bevington: "Hyperphosphatemia, Phosphoprotein Phosphatases, and Microparticle Release in Vascular Endothelial Cells." in: Journal of the American Society of Nephrology : JASN, Vol. 26, Issue 9, pp. 2152-62, (2015) (PubMed).

Target Details for tau

Target: tau (tau Protein)

Alternative Name: Tau (tau Products)

Synonyms: DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, tau antibody, PHF-tau antibody, microtubule associated protein tau antibody, MAPT antibody

Background: Tau is a microtubule-associated phosphoprotein (MAP) localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, ranging from 352 to 441 amino acids. The tau variants differ from each other with the presence of either three or four repeat-regions near the carboxy-terminal and the absence or presence of one or two inserts near the amino-terminal.Tau is hyperphosphorylated by ERK, GSK-3beta, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404, 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein found in the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimers disease (AD). The PHFs are also found in the lesions of other central nervous system disorders