E Tag antibody (HRP)

Catalog No. ABIN1842498

Product Details anti-E Tag Antibody (HRP)

Target: E Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Sequence: GAPVPYPDPL EPR

Specificity: Anti-E-tag [HRP] Polyclonal Antibody recognizes E tagged fusion proteins.

Characteristics: Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags.

Purification: Immunoaffinity chromatography

Immunogen: E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

Target Details for E Tag

Target: E Tag

Abstract: E Tag Products

Target Type: Tag