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CD4 antibody (FITC)

CD4 Reactivity: Guinea Pig FACS Host: Mouse Monoclonal CT7 FITC

Catalog No. ABIN182094

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Reactivity
333

199

111

48

24

20

19

18

18

12

10

10

10

10

10

9

9

9

8

5

4

3

1

1

1

1

1
Guinea Pig
Host
338

149

109

3

3

1
Mouse
Compatible Secondaries
Clonality
459

138

3
Monoclonal
Conjugate
208

72

63

45

33

13

12

11

9

9

9

8

5

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD4 antibody is conjugated to FITC
Application
484

193

125

102

92

74

69

42

39

34

33

26

10

9

9

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

Purification

Affinity chromatography on Protein G

Immunogen

Guinea pig peritoneal T-cells. Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line

Clone

CT7

Isotype

IgG1

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CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

Application Notes

Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole Guinea Pig peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1 mg/mL

Buffer

PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Expiry Date

12 months
Target

CD4

Alternative Name

CD4 (CD4 Products)

Background

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, T4/Leu-3

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events