This antibody recognises beta-glucan receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
Purification
Affinity chromatography on Protein G
Immunogen
Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
CLEC7A
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
CLEC7A
(C-Type Lectin Domain Family 7, Member A (CLEC7A))
Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.Synonyms: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1