This V5 Epitope Tag antibody is conjugated to FITC
Application
Immunofluorescence (IF)
Sequence
GKPIPNPLLG LDST
Purification
Affinity chromatography on Protein G
Immunogen
Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Immunofluorescence: 1/100, Membrane permeabilisation with 0.5 % IGEPAL is required for this application. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is manufactured under an exclusive license from the University of St. Andrews,UK.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
Containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.