C5AR1 antibody (N-Term)
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- Target See all C5AR1 Antibodies
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
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Binding Specificity
- AA 1-31, N-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This C5AR1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognises the C5a receptor (C5aR) CD88. Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31). Clone P12/1 does not inhibit the binding of C5a to its receptor. We recommend the use of SM1182A for functional studies.
- Purification
- Affinity Chromatography on Protein G
- Immunogen
- C5aR - peptide: Met1 - Asn31. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
- Clone
- P12-1
- Isotype
- IgG2a
- Top Product
- Discover our top product C5AR1 Primary Antibody
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anti-Complement Component 5a Receptor 1 (C5AR1) (C-Term) antibody
Verified C5AR1 Reactivity: Human WB, FACS, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibodyC5AR1 Reactivity: Human, Cow, Rabbit, Ferret WB, FACS, IHC (p), IP Host: Mouse Monoclonal S5-1 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4E2 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 250-350) antibodyC5AR1 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-36) antibodyC5AR1 Reactivity: Human WB, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (FITC)C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 FITC
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 175-201) antibodyC5AR1 Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB24276 unconjugated
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (APC)C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 APC
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 15-27) antibody (PE)C5AR1 Reactivity: Human, Cow, Rabbit, Ferret FACS Host: Mouse Monoclonal S5-1 PE
anti-Complement Component 5a Receptor 1 (C5AR1) (AA 1-350) antibodyC5AR1 Reactivity: Human WB, PLA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot. Immunohistochemistry on frozen section. Immunohistochemistry on paraffin section. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 5 x 10e5 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
- Alternative Name
- CD88 / C5R1 (C5AR1 Products)
- Background
- C5a Anaphylatoxin Receptor mediates the effects of one of the most potent chemotactic agents known, in a wide variety of inflammatory cell types. C5a receptor 1 has been implicated in asthma. CD88 is predominantly expressed on cells of the myeloid lineage.Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1
- Gene ID
- 728
- NCBI Accession
- NP_001727
- UniProt
- P21730
- Pathways
- Complement System
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