Complement C8 antibody
C8
Reactivity: Human
FACS, WB, EIA, IHC (fro)
Host: Mouse
Monoclonal
056B-373
unconjugated
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- Target See all Complement C8 (C8) products
- Complement C8 (C8)
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Complement C8 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognizes complement component 8 (C8), a 151 kDa member of the complement C6/C7/C8/C9 family, present in blood serum.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Characteristics
- Synonyms: Complement component C8, Complement 8
- Purification
- Affinity Chromatography on Protein A
- Immunogen
- Purified Human C8. Genename: C8
- Clone
- 056B-373
- Isotype
- IgG1
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- Application Notes
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ELISA. Western Blot. Flow Cytometry. Immunohistochemistry on Frozen Sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Borate buffered saline pH 8.2-8.4 containing 0.02 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Complement C8 (C8)
- Alternative Name
- Complement C8 (C8 Products)
- Background
- C8 is a terminal component of the complement system, part of both the complement membrane attack complex (MAC), and important to MAC assembly. C8 binds to the C5b-7 complex, anchored to the membrane, creating C5b-8. C5b-8 binds C9 and catalyses the polymerization of C9 Molecules to form C5-b9 (MAC). C8 is thought to contain lipid binding sites, facilitating the insertion of MAC into the membrane. Defects in the alpha chain of C8 can result in complement C8 deficiency type I. Furthermore, C8 deficiencies can cause recurring bacterial infections, in particular from Neisseria meningitides.Synonyms: Complement 8, Complement component C8
- Gene ID
- 732
- UniProt
- P07358
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