Soluble Terminal Complement Complex (sC5b-9) antibody
sC5b-9
Reactivity: Human
FACS, IHC (fro), WB, EIA
Host: Mouse
Monoclonal
3R2-0
unconjugated
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- Target
- Soluble Terminal Complement Complex (sC5b-9)
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Enzyme Immunoassay (EIA)
- Characteristics
- Synonyms: Terminal complement complex, Membrane attack complex
- Purification
- Affinity Chromatography on Protein A
- Clone
- 3R2-0
- Isotype
- IgG2b
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- Application Notes
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ELISA. Western Blot. Flow Cytometry. Immunohistochemistry on frozen sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Borate buffered saline, pH 8.4 containing 0.09 % Sodium Azide as preservative
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Soluble Terminal Complement Complex (sC5b-9)
- Alternative Name
- Complement SC5b-9 (TCC / MAC)
- Background
- Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation.Synonyms: Membrane attack complex, Terminal complement complex
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