CD79a antibody (AA 202-216)

Catalog No. ABIN181100

Product Details anti-CD79a Antibody

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Binding Specificity: AA 202-216

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey, Chicken, Horse, Rabbit, Guinea Pig, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognizes an epitope within the cytoplasmic domain of CD79a.

Cross-Reactivity (Details): Species reactivity (tested):Human, American Bison, Fallow Deer, Canine, Monkey, Pig, Horse, Bovine, Guinea Pig, Rat, Mouse, Rabbit, Chicken.

Characteristics: Synonyms: IGA, MB1, B-Cell marker, B-cell antigen receptor complex-associated protein alpha-chain,MB-1 membrane glycoprotein, Surface IgM-associated protein, Membrane-boundimmunoglobulin-associated protein

Purification: Affinity Chromatography on Protein G

Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1. Spleencells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line. Genename: CD79a

Clone: HM57

Isotype: IgG1

Application Details

Application Notes: Western Blot. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval usingheat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 isrecommended for this purpose. Recommended Positive Control: Human tonsilFlow Cytometry: 1/10 - 1/25, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: CD79a (CD79A Products)

Synonyms: CD79A antibody, Ig-alpha antibody, Iga antibody, Igalpha antibody, Ly-54 antibody, Ly54 antibody, mb-1 antibody, IGA antibody, MB-1 antibody, IG-alpha antibody, CD79a molecule antibody, CD79A antigen (immunoglobulin-associated alpha) antibody, Cd79a molecule-like antibody, CD79A antibody, Cd79a antibody, Cd79al antibody

Background: CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin.Synonyms: B-Cell marker, B-cell antigen receptor complex-associated protein alpha-chain, IGA, MB-1 membrane glycoprotein, MB1, Membrane-bound immunoglobulin-associated protein, Surface IgM-associated protein

Gene ID: 973

UniProt: P11912

Pathways: BCR Signaling