This antibody recognises the CD301 cell surface protein. In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Clone ER-MP23 is reported to block the function of murine CD301. The antibody binds both MGL1 and MGL2 homologues.
Characteristics
Synonyms: CLECSF13, CLECSF14, HML, C-type lectin domain family 10 member A, C-type lectinsuperfamily member 14, Macrophage lectin 2
Purification
Affinity Chromatography on Protein G
Immunogen
Balb/c macrophage precursor cell hybrids. Cells from immunised rats were fused with cells of the rat Y3-Ag1. 2. 3 myeloma cell line.
CLEC10A
Reactivity: Human
WB, FACS
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Lederle, Hartenstein, Meides, Kunzelmann, Werb, Angel, Mueller: "MMP13 as a stromal mediator in controlling persistent angiogenesis in skin carcinoma." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1175-84, (2010) (PubMed).
Target
CLEC10A
(C-Type Lectin Domain Family 10, Member A (CLEC10A))
CLEC10A antibody, CD301 antibody, CLECSF13 antibody, CLECSF14 antibody, HML antibody, HML2 antibody, MGL antibody, CD301a antibody, M-ASGP-BP-1 antibody, Mgl antibody, Mgl1 antibody, Mgll antibody, C-type lectin domain family 10 member A antibody, C-type lectin domain containing 10A antibody, C-type lectin domain family 10, member A antibody, LOC607405 antibody, CLEC10A antibody, Clec10a antibody
Background
In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated.Synonyms: C-type lectin domain family 10 member A, C-type lectin superfamily member 14, CLECSF13, CLECSF14, HML, Macrophage lectin 2