Podoplanin antibody

Catalog No. ABIN180856

Product Details anti-Podoplanin Antibody

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The monoclonal antibody will detect Human Podoplanin on the surface of lymphatic endothelial cells and kidney podocytes. Therefore, it will be a useful tool to study lymphatic vessel density in and around Human tumors. Does not react with Mouse.

Characteristics: Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025

Purification: Purified

Clone: NZ-1

Isotype: IgG2a

Application Details

Application Notes: FACS (1/200). Western blot (>/= 1/200). Immunoprecipitation: 1/50. Immunostaining/Immunohistochemistry on Frozen and Paraffin Sections (1/200). Suitablefor Formalin-Fixed Paraffin Embedded Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in working aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

References for anti-Podoplanin antibody (ABIN180856)

Martín-Villar, Fernández-Muñoz, Parsons, Yurrita, Megías, Pérez-Gómez, Jones, Quintanilla: "Podoplanin associates with CD44 to promote directional cell migration." in: Molecular biology of the cell, Vol. 21, Issue 24, pp. 4387-99, (2011) (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Background: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 10630

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport