CD74 antibody

Catalog No. ABIN180804

Product Details anti-CD74 Antibody

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody recognizes Human CD74, a type II transmembrane protein, which is also known as the MHC class II associated invariant chain. The Bu45 antibody recognizes 33, 35 and 41 kDa isoforms of CD74.

Characteristics: Synonyms: p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DRantigens-associated invariant chain, Ia antigen-associated invariant chain

Purification: Affinity chromatography on Protein G

Immunogen: B lymphoblastoid cell line HFB1. Spleen cells from immunised mice were fused with cells ofthe Ag8. 653 myeloma cell line. Genename: CD74

Clone: Bu45

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/25 - 1/50,use 10 μL of the suggested working dilution to label 1x106 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at +4 °C or at -20 °C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 (CD74 Products)

Background: The CD74 Molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 Molecule, which is distal from the C-terminus.Synonyms: DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints