Moesin antibody

Catalog No. ABIN1803989

Product Details anti-Moesin Antibody

Target: Moesin (MSN)

Reactivity: Human, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Moesin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Recognizes 78kD moesin. Weakly recognizes 80kD radixin. Detects most of the proteolytic fragments of moesin. Does not react with talin or ezrin.

Purification: Protein G purified

Immunogen: Moesin from cow uterus.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: Denatured only. 2 μg/mg protein lysate using Protein G. Western Blot: 1-2 μg/mL for 2 hours at RT. Positive control: Raji cells, placenta.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Moesin

Target: Moesin (MSN)

Alternative Name: MSN / Moesin (MSN Products)

Background: Name/Gene ID: MSN

Synonyms: MSN, Moesin

Gene ID: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization