FcRn antibody

Catalog No. ABIN1774763

Product Details anti-FcRn Antibody

Key Features:

• High-quality FcRn antibody (Clone DVN24) for the detection of FcRn.
• Highly specific for human FcRn, blocks the binding of IgG.
• PBS-only, preservative free
• 2 PubMed references available for this FcRn antibody.

Target: FcRn (IgG receptor FcRn (FcRn))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FcRn antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Cell-ELISA (cELISA)

Characteristics: based on recognition of the complete native protein expressed on transfected mammalian cells

Purification: Purified, Protein G

Immunogen: hFcRn

Clone: DVN24

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: 1.2 μg/10^6 cells ELISA: 1:200 - 1:400CELISA: 1:200 - 1:400For each application a titration should be performed to determine the optimal concentration.

Restrictions: For Research Use only

Handling

Buffer: PBS pH 7.2

Handling Advice: avoid repeated freezing and thawing

Storage: 4 °C

References for anti-FcRn antibody (ABIN1774763)

Xu, He, Momben-Abolfath, Vertrees, Li, Norton, Struble: "Zika Virus Infection and Antibody Neutralization in FcRn Expressing Placenta and Engineered Cell Lines." in: Vaccines, Vol. 10, Issue 12, (2022) (PubMed).

Hubbard, Pyzik, Rath, Kozicky, Sand, Gandhi, Grevys, Foss, Menzies, Glickman, Fiebiger, Roopenian, Sandlie, Andersen, Sly, Baker, Blumberg: "FcRn is a CD32a coreceptor that determines susceptibility to IgG immune complex-driven autoimmunity." in: The Journal of experimental medicine, Vol. 217, Issue 10, (2021) (PubMed).

Cines, Zaitsev, Rauova, Rux, Stepanova, Krishnaswamy, Sarkar, Kowalska, Zhao, Mast, Blumberg, McCrae, Poncz, Hubbard, Pyzik, Blumberg: "FcRn augments induction of tissue factor activity by IgG-containing immune complexes." in: Blood, Vol. 135, Issue 23, pp. 2085-2093, (2021) (PubMed).

Grevys, Nilsen, Sand, Daba, Øynebråten, Bern, McAdam, Foss, Schlothauer, Michaelsen, Christianson, Roopenian, Blumberg, Sandlie, Andersen: "A human endothelial cell-based recycling assay for screening of FcRn targeted molecules." in: Nature communications, Vol. 9, Issue 1, pp. 621, (2018) (PubMed).

Christianson, Sun, Akilesh, Pesavento, Proetzel, Roopenian: "Monoclonal antibodies directed against human FcRn and their applications." in: mAbs, Vol. 4, Issue 2, pp. 208-16, (2014) (PubMed).

Sand, Dalhus, Christianson, Bern, Foss, Cameron, Sleep, Bjørås, Roopenian, Sandlie, Andersen: "Dissection of the neonatal Fc receptor (FcRn)-albumin interface using mutagenesis and anti-FcRn albumin-blocking antibodies." in: The Journal of biological chemistry, Vol. 289, Issue 24, pp. 17228-39, (2014) (PubMed).

Target Details for FcRn

Target: FcRn (IgG receptor FcRn (FcRn))

Abstract: FcRn Products

Synonyms: FCRN antibody, alpha-chain antibody, FcRn antibody, Fc fragment of IgG receptor and transporter antibody, Fc receptor, IgG, alpha chain transporter antibody, FCGRT antibody, Fcgrt antibody

Background: The MHC class I-like Fc receptor (FcRn) is an intracellular trafficking Fc receptor that is uniquely responsible for the extended serum half-life of antibodies of the IgG subclass and their ability to transport across cellular barriers. By performing these functions, FcRn affects numerous facets of antibody biology and pathobiology. Its critical role in controlling IgG pharmacokinetics has been leveraged for the design of therapeutic antibodies and related biologics. FcRn also traffics serum albumin and is responsible for the enhanced pharmacokinetic properties of albumin-conjugated therapeutics.
Synonyms: IgG receptor FcRn large subunit p51, Neonatal Fc receptor, FCGRT

UniProt: P55899

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process