Nestin antibody

Catalog No. ABIN1742564

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for human nestin.

Purification: purified IgG

Immunogen: Enriched fraction of the neuronal cytoskeleton.

Clone: JP39

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000

Comment:

Differences between clone JP 63 and clone JP39 in application or specificity not determined yet.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in TRIS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)