ZYX antibody (AA 352-357)

Catalog No. ABIN1742558

Product Details anti-ZYX Antibody

Target: ZYX (Zyxin (ZYX))

Binding Specificity: AA 352-357

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZYX antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for zyxin.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant human zyxin. Epitope TLKEVE (aa 352-357 of human zyxin)

Clone: 164D4

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 100 up to 1 : 2000 (AP staining)
ICC: 1 : 100
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-ZYX antibody (ABIN1742558)

Missirlis, Haraszti, Scheele, Wiegand, Diaz, Neubauer, Rechenmacher, Kessler, Spatz: "Substrate engagement of integrins α5β1 and αvβ3 is necessary, but not sufficient, for high directional persistence in migration on fibronectin." in: Scientific reports, Vol. 6, pp. 23258, (2016) (PubMed).

Gadhari, Charnley, Marelli, Brugger, Chiquet: "Cell shape-dependent early responses of fibroblasts to cyclic strain." in: Biochimica et biophysica acta, Vol. 1833, Issue 12, pp. 3415-25, (2013) (PubMed).

Delaroche, Cantrelle, Subra, Van Heijenoort, Guittet, Jiao, Blanchoin, Chassaing, Lavielle, Auclair, Sagan: "Cell-penetrating peptides with intracellular actin-remodeling activity in malignant fibroblasts." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7712-21, (2010) (PubMed).

Low, Mukhina, Srinivas, Ng, Murata-Hori: "Domain analysis of alpha-actinin reveals new aspects of its association with F-actin during cytokinesis." in: Experimental cell research, Vol. 316, Issue 12, pp. 1925-34, (2010) (PubMed).

Mori, Nakagami, Koibuchi, Miura, Takami, Koriyama, Hayashi, Sabe, Mochizuki, Morishita, Kaneda: "Zyxin mediates actin fiber reorganization in epithelial-mesenchymal transition and contributes to endocardial morphogenesis." in: Molecular biology of the cell, Vol. 20, Issue 13, pp. 3115-24, (2009) (PubMed).

Vollenbröker, George, Wolfgart, Saleem, Pavenstädt, Weide: "mTOR regulates expression of slit diaphragm proteins and cytoskeleton structure in podocytes." in: American journal of physiology. Renal physiology, Vol. 296, Issue 2, pp. F418-26, (2009) (PubMed).

Vervenne, Crombez, Delvaux, Janssens, Van de Ven, Petit: "Targeted disruption of the mouse Lipoma Preferred Partner gene." in: Biochemical and biophysical research communications, Vol. 379, Issue 2, pp. 368-73, (2009) (PubMed).

Guo, Wang: "Retrograde fluxes of focal adhesion proteins in response to cell migration and mechanical signals." in: Molecular biology of the cell, Vol. 18, Issue 11, pp. 4519-27, (2007) (PubMed).

Coutts, MacKenzie, Griffith, Black: "TES is a novel focal adhesion protein with a role in cell spreading." in: Journal of cell science, Vol. 116, Issue Pt 5, pp. 897-906, (2003) (PubMed).

Garvalov, Higgins, Sutherland, Zettl, Scaplehorn, Köcher, Piddini, Griffiths, Way: "The conformational state of Tes regulates its zyxin-dependent recruitment to focal adhesions." in: The Journal of cell biology, Vol. 161, Issue 1, pp. 33-9, (2003) (PubMed).

Rottner, Krause, Gimona, Small, Wehland: "Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions." in: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3103-13, (2001) (PubMed).

Target Details for ZYX

Target: ZYX (Zyxin (ZYX))

Alternative Name: Zyxin (ZYX Products)

Synonyms: BcDNA:LD06023 antibody, CG32018 antibody, DLim-1 antibody, DmLIM-1 antibody, Dmel\\CG32018 antibody, Lim102EF antibody, ZYX102 antibody, Zyx102 antibody, Zyx102EF antibody, lim antibody, zyx102 antibody, zyxin antibody, zgc:171680 antibody, ZYX antibody, zyx antibody, MGC145270 antibody, ESP-2 antibody, HED-2 antibody, 9530098H06Rik antibody, R75157 antibody, Zyxin antibody, zyxin antibody, zyxin S homeolog antibody, zyx-1 antibody, Zyx antibody, zyx antibody, ZYX antibody, EDI_201420 antibody, zyx.S antibody