TUBB3 antibody (AA 443-450)

Catalog No. ABIN1742554

Product Details anti-TUBB3 Antibody

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Binding Specificity: AA 443-450

Reactivity: Human, Mouse, Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for beta3-tubulin.

Purification: antiserum

Immunogen: Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200

Comment:

IHC: For best results we recommend antigen retrieval with pepsin according to Lorincz A & Nusser Z (2008).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-TUBB3 antibody (ABIN1742554)

Matthäus, Haddjeri, Sánchez, Martí, Bahri, Rovera, Schloss, Lau: "The allosteric citalopram binding site differentially interferes with neuronal firing rate and SERT trafficking in serotonergic neurons." in: European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, Vol. 26, Issue 11, pp. 1806-1817, (2016) (PubMed).

Andres, Keyser, Petrali, Benton, Hubbard, McNutt, Ray: "Morphological and functional differentiation in BE(2)-M17 human neuroblastoma cells by treatment with Trans-retinoic acid." in: BMC neuroscience, Vol. 14, pp. 49, (2013) (PubMed).

Lau, Heimann, Bartsch, Schloss, Weber: "Nongenomic, glucocorticoid receptor-mediated regulation of serotonin transporter cell surface expression in embryonic stem cell derived serotonergic neurons." in: Neuroscience letters, Vol. 554, pp. 115-20, (2013) (PubMed).

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: beta3-Tubulin (TUBB3) (TUBB3 Products)

Synonyms: CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, TUBB3 antibody, 3200002H15Rik antibody, M(beta)3 antibody, M(beta)6 antibody, tubb3 antibody, tubulin beta 3 class III antibody, tubulin, beta 3 class III antibody, tubulin beta 3 class III L homeolog antibody, beta-tubulin 3 antibody, tubulin beta-3 chain antibody, beta tubulin 3 antibody, TUBB3 antibody, Tubb3 antibody, tubb3 antibody, tubb3.L antibody, LOC100581245 antibody, tub3 antibody

Background: Synonyms: TuJ1

Pathways: Microtubule Dynamics, M Phase