Tubulin delta 2 (AA 443-449) antibody
Reactivity: Human, Mammalian, Mouse, Rat
WB, ICC
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Tubulin delta 2
- Binding Specificity
- AA 443-449
- Reactivity
- Human, Mammalian, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC)
- Specificity
- Specific for delta2-tubulin.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Synthetic peptide EGEEEGE (aa 443-449 of rat alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
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anti-Tubulin delta 2 (C-Term) antibody
Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 500
IHC: not tested yet - Comment
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This antibody has been cross-adsorbed against peptides corresponding to the C-termini of glu- and tyr-tubulin. ICC: Requires long permeabilization with 0.3% Tritonx100 for 30 min.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- Target
- Tubulin delta 2
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