CHGB antibody (AA 407-677)

Catalog No. ABIN1742518

Product Details anti-CHGB Antibody

Target: CHGB (Chromogranin B (Secretogranin 1) (CHGB))

Binding Specificity: AA 407-677

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHGB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific chromogranin B.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein of mouse chromogranin B (aa 407-677).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC-P: 1 : 200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-CHGB antibody (ABIN1742518)

Alvarez-Saavedra, De Repentigny, Yang, OMeara, Yan, Hashem, Racacho, Ioshikhes, Bulman, Parks, Kothary, Picketts: "Voluntary Running Triggers VGF-Mediated Oligodendrogenesis to Prolong the Lifespan of Snf2h-Null Ataxic Mice." in: Cell reports, Vol. 17, Issue 3, pp. 862-875, (2016) (PubMed).

Farina, van de Bospoort, He, Persoon, van Weering, Broeke, Verhage, Toonen: "CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons." in: eLife, Vol. 4, (2015) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Target Details for CHGB

Target: CHGB (Chromogranin B (Secretogranin 1) (CHGB))

Alternative Name: Chromogranin B (CHGB Products)

Synonyms: SCG1 antibody, Scg-1 antibody, CGB antibody, CHGB antibody, si:ch73-355e4.2 antibody, si:cu075930.2 antibody, chromogranin B antibody, CHGB antibody, Chgb antibody, chgb antibody

Background: Synonyms: CgB