GABRB2 antibody (AA 343-430)
-
- Target See all GABRB2 Antibodies
- GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))
-
Binding Specificity
- AA 343-430
-
Reactivity
- Rat, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This GABRB2 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- Specific for GABA-A receptor beta2.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Recombinant protein (aa 343-430 in mouse) of the unprocessed precursor forced into a loop-shaped structure.
- Top Product
- Discover our top product GABRB2 Primary Antibody
-
-
- Application Notes
-
WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500 - Comment
-
WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
-
-
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells." in: eLife, Vol. 5, (2016) (PubMed).
: "
-
Similar GABAA receptor subunit composition in somatic and axon initial segment synapses of hippocampal pyramidal cells." in: eLife, Vol. 5, (2016) (PubMed).
-
- Target
- GABRB2 (gamma-aminobutyric Acid (GABA) A Receptor, beta 2 (GABRB2))
- Alternative Name
- GABA-A Receptor Beta2 (GABRB2 Products)
- Synonyms
- GABRB2 antibody, GARB2 antibody, fj59e04 antibody, gabaabeta2 antibody, wu:fj59e04 antibody, zgc:136311 antibody, GABARB antibody, AI834970 antibody, C030002O17Rik antibody, C030021G16Rik antibody, Gabrab2 antibody, Gabrb-2 antibody, gamma-aminobutyric acid type A receptor beta2 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, beta 2 antibody, gamma-aminobutyric acid type A receptor beta 2 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit beta 2 antibody, GABRB2 antibody, gabrb2 antibody, Gabrb2 antibody
- Background
- Synonyms: GABA(A) R beta2
- Pathways
- Sensory Perception of Sound, Synaptic Membrane
-