LRRC32
Reactivity: Human
WB
Host: Mouse
Monoclonal
50G10
unconjugated
Application Notes
WB: not recommended IP: not tested yet ICC: not tested yet IHC: not tested yet FACS: 1 : 100 up to 1 : 1000
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS, 0.02% sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage
-20 °C
Storage Comment
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Cuende, Liénart, Dedobbeleer, van der Woning, De Boeck, Stockis, Huygens, Colau, Somja, Delvenne, Hannon, Baron, Dumoutier, Renauld, De Haard, Saunders, Coulie, Lucas: "Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo." in: Science translational medicine, Vol. 7, Issue 284, pp. 284ra56, (2015) (PubMed).
Rappl, Pabst, Riemann, Schmidt, Wickenhauser, Schütte, Hombach, Seliger, Grohé, Abken: "Regulatory T cells with reduced repressor capacities are extensively amplified in pulmonary sarcoid lesions and sustain granuloma formation." in: Clinical immunology (Orlando, Fla.), Vol. 140, Issue 1, pp. 71-83, (2011) (PubMed).
Probst-Kepper, Geffers, Kröger, Viegas, Erck, Hecht, Lünsdorf, Roubin, Moharregh-Khiabani, Wagner, Ocklenburg, Jeron, Garritsen, Arstila, Kekäläinen, Balling, Hauser, Buer, Weiss: "GARP: a key receptor controlling FOXP3 in human regulatory T cells." in: Journal of cellular and molecular medicine, Vol. 13, Issue 9B, pp. 3343-57, (2010) (PubMed).