LRRC32 antibody (AA 23-612)

Catalog No. ABIN1742449

Product Details anti-LRRC32 Antibody

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Binding Specificity: AA 23-612

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRRC32 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for GARP.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Recombinant ectodomain of human GARP (aa 23-612).

Clone: 50G10

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: not tested yet

Comment:

Skimmed milk is required for efficient blocking.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-LRRC32 antibody (ABIN1742449)

Probst-Kepper, Geffers, Kröger, Viegas, Erck, Hecht, Lünsdorf, Roubin, Moharregh-Khiabani, Wagner, Ocklenburg, Jeron, Garritsen, Arstila, Kekäläinen, Balling, Hauser, Buer, Weiss: "GARP: a key receptor controlling FOXP3 in human regulatory T cells." in: Journal of cellular and molecular medicine, Vol. 13, Issue 9B, pp. 3343-57, (2010) (PubMed).

Target Details for LRRC32

Target: LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

Alternative Name: GARP (LRRC32 Products)

Synonyms: D11S833E antibody, GARP antibody, AI426318 antibody, D11S833Eh antibody, D7H11S833E antibody, EG434215 antibody, Garp antibody, leucine rich repeat containing 32 antibody, LRRC32 antibody, Lrrc32 antibody

Background: Synonyms: LRRC 32

Pathways: Activated T Cell Proliferation