Abeta 1-42 antibody (AA 37-42)

Catalog No. ABIN1742444

Product Details anti-Abeta 1-42 Antibody

Target: Abeta 1-42 (Amyloid beta 1-42 (Abeta 1-42))

Binding Specificity: AA 37-42

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Abeta 1-42 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: Specific for Abeta 42

Cross-Reactivity (Details): weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide corresponding to aa 37-42 of Abeta peptide 42 coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (ECL detection)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500
IHC-P: 1 : 100
ELISA: yes, suitable only as capture antibody, ABIN1742439 is recommended detector antibody for sandwich-ELISA (protocol)

Comment:

WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Abeta 1-42 antibody (ABIN1742444)

Wang, Wu, Anand, Karthivashan, Phukan, Yang, Thinakaran, Westaway, Kar: "Significance of cytosolic cathepsin D in Alzheimer's disease pathology: Protective cellular effects of PLGA nanoparticles against β-amyloid-toxicity." in: Neuropathology and applied neurobiology, Vol. 46, Issue 7, pp. 686-706, (2021) (PubMed).

Hüttenrauch, Baches, Gerth, Bayer, Weggen, Wirths: "Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 44, Issue 4, pp. 1291-302, (2015) (PubMed).

Guzmán, Bouter, Richard, Lannfelt, Ingelsson, Paetau, Verkkoniemi-Ahola, Wirths, Bayer: "Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease." in: Molecular neurodegeneration, Vol. 9, pp. 13, (2014) (PubMed).

Reinert, Martens, Huettenrauch, Kolbow, Lannfelt, Ingelsson, Paetau, Verkkoniemi-Ahola, Bayer, Wirths: "Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 4, pp. 871-81, (2014) (PubMed).

Christensen, Huettenrauch, Mitkovski, Pradier, Wirths: "Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal A? accumulation." in: Frontiers in aging neuroscience, Vol. 6, pp. 139, (2014) (PubMed).

Bouter, Dietrich, Wittnam, Rezaei-Ghaleh, Pillot, Papot-Couturier, Lefebvre, Sprenger, Wirths, Zweckstetter, Bayer: "N-truncated amyloid β (Aβ) 4-42 forms stable aggregates and induces acute and long-lasting behavioral deficits." in: Acta neuropathologica, Vol. 126, Issue 2, pp. 189-205, (2013) (PubMed).

Target Details for Abeta 1-42

Target: Abeta 1-42 (Amyloid beta 1-42 (Abeta 1-42))

Alternative Name: Abeta 42 (Abeta 1-42 Products)

Synonyms: amyloid beta precursor protein antibody, App antibody, APP antibody

Background: Synonyms: Beta-App, ABPP