Ribeye (AA 95-207) antibody
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- Target
- Ribeye
- Binding Specificity
- AA 95-207
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Reactivity
- Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- This antibody recognizes only ribeye and not CtBP 2.
- Cross-Reactivity (Details)
- Other species not tested yet.
- Characteristics
- A scaffolding protein of the ribbon synapse
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Recombinant protein corresponding to AA 95 to 207 from rat Ribeye (UniProt Id: Q9EQH5-2)
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- Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion." in: Development (Cambridge, England), Vol. 145, Issue 3, (2018) (PubMed).
: "Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 1, pp. 222-34, (2016) (PubMed).
: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).
: "Localization of kainate receptors in inner and outer hair cell synapses." in: Hearing research, Vol. 314, pp. 20-32, (2014) (PubMed).
: "In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses." in: Frontiers in cellular neuroscience, Vol. 8, pp. 259, (2014) (PubMed).
: "SLITRK6 mutations cause myopia and deafness in humans and mice. ..." in: The Journal of clinical investigation, Vol. 123, Issue 5, pp. 2094-102, (2013) (PubMed).
: "Rod photoreceptor ribbon synapses in DBA/2J mice show progressive age-related structural changes." in: PLoS ONE, Vol. 7, Issue 9, pp. e44645, (2012) (PubMed).
: "Differential spatial expression and subcellular localization of CtBP family members in rodent brain." in: PLoS ONE, Vol. 7, Issue 6, pp. e39710, (2012) (PubMed).
: "Absence of functional active zone protein Bassoon affects assembly and transport of ribbon precursors during early steps of photoreceptor synaptogenesis." in: European journal of cell biology, Vol. 89, Issue 6, pp. 468-75, (2010) (PubMed).
: "
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RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion." in: Development (Cambridge, England), Vol. 145, Issue 3, (2018) (PubMed).
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- Target
- Ribeye
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