VAMP5 antibody (AA 1-70)

Catalog No. ABIN1742369

Product Details anti-VAMP5 Antibody

Target: VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))

Binding Specificity: AA 1-70

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Specific for myobrevin.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant mouse myobrevin (aa 1-70).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC-P: 1 : 200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-VAMP5 antibody (ABIN1742369)

Ikezawa, Tajika, Ueno, Murakami, Inoue, Yorifuji: "Loss of VAMP5 in mice results in duplication of the ureter and insufficient expansion of the lung." in: Developmental dynamics : an official publication of the American Association of Anatomists, (2017) (PubMed).

Sadler, Bryant, Gould: "Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking." in: Molecular biology of the cell, Vol. 26, Issue 3, pp. 530-6, (2015) (PubMed).

Takahashi, Tajika, Khairani, Ueno, Murakami, Yorifuji: "The localization of VAMP5 in skeletal and cardiac muscle." in: Histochemistry and cell biology, Vol. 139, Issue 4, pp. 573-82, (2013) (PubMed).

Rose, Jeppesen, Kiens, Richter: "Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 297, Issue 5, pp. R1228-37, (2009) (PubMed).

Target Details for VAMP5

Target: VAMP5 (Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5))

Alternative Name: Myobrevin (VAMP5 Products)

Synonyms: AF119384 antibody, Camp antibody, vesicle associated membrane protein 5 antibody, vesicle-associated membrane protein 5 antibody, VAMP5 antibody, vamp5 antibody, Vamp5 antibody

Background: Synonyms: VAMP 5