WB: 1 : 1000 (AP staining) ICC: 1 : 100 up to 1 : 500
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Ferru-Clément, Fresquet, Norez, Métayé, Becq, Kitzis, Thoreau: "Involvement of the Cdc42 pathway in CFTR post-translational turnover and in its plasma membrane stability in airway epithelial cells." in: PLoS ONE, Vol. 10, Issue 3, pp. e0118943, (2015) (PubMed).