HOMER3 antibody (AA 1-177)

Catalog No. ABIN1742342

Product Details anti-HOMER3 Antibody

Target: HOMER3 (Homer Homolog 3 (Drosophila) (HOMER3))

Binding Specificity: AA 1-177

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HOMER3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: AA 122-177, unique for homer 3, were used for affinity purification. According to Soloviev et al. (2000), they are present in isoform a, partially present b, and absent from c and d.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Purified recombinant protein of rat homer 3 (aa 1-177).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 250
IHC: 1 : 500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-HOMER3 antibody (ABIN1742342)

Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).

Target Details for HOMER3

Target: HOMER3 (Homer Homolog 3 (Drosophila) (HOMER3))

Alternative Name: Homer 3 (HOMER3 Products)

Synonyms: zgc:55557 antibody, HOMER-3 antibody, VESL3 antibody, AW146114 antibody, Vesl-3 antibody, homer scaffolding protein 3b antibody, homer scaffolding protein 3 antibody, homer3b antibody, HOMER3 antibody, Homer3 antibody

Background: Synonyms: VesL 3