CACNA1B antibody (AA 1921-2222)
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- Target See all CACNA1B Antibodies
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
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Binding Specificity
- AA 1921-2222
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CACNA1B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- Specific for Ca2+ channel alpha-1E.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).
- Top Product
- Discover our top product CACNA1B Primary Antibody
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- Application Notes
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WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 - Comment
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WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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- Target
- CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))
- Alternative Name
- Ca2+ Channel (CACNA1B Products)
- Synonyms
- BIII antibody, CACNL1A5 antibody, CACNN antibody, Cav2.2 antibody, AW050276 antibody, AW060892 antibody, AW822256 antibody, Cchn1a antibody, alpha(1B) antibody, calcium voltage-gated channel subunit alpha1 B antibody, calcium channel, voltage-dependent, N type, alpha 1B subunit antibody, CACNA1B antibody, Cacna1b antibody
- Background
- Synonyms: Cav2.3
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