GLRB antibody (AA 336-355, AA 336-455)

Catalog No. ABIN1742318

Product Details anti-GLRB Antibody

Target: GLRB (Glycine Receptor, beta (GLRB))

Binding Specificity: AA 336-355, AA 336-455

Reactivity: Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLRB antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Specific for the glycine receptor beta-subunit.

Purification: purified IgG

Immunogen: Recombinant protein (aa 336-455 of rat GlyR beta) Epitope aa 336-355

Clone: 299E7

Isotype: IgG1

Application Details

Application Notes: WB: not recommended
IP: not tested yet
IHC: 1 : 500 up to 1 : 1000

Comment:

WB: Detection of overexpressed protein is possible. Endogenous levels are hardly detectable. IHC: Mild fixation for 10-15 min with cold 2 % PFA solution is recommended. Include 0,5 % triton X 100 in all blocking and antibody incubation steps.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-GLRB antibody (ABIN1742318)

Weltzien, Puller, OSullivan, Paarmann, Betz: "Distribution of the glycine receptor β-subunit in the mouse CNS as revealed by a novel monoclonal antibody." in: The Journal of comparative neurology, Vol. 520, Issue 17, pp. 3962-81, (2012) (PubMed).

Target Details for GLRB

Target: GLRB (Glycine Receptor, beta (GLRB))

Alternative Name: Glycine Receptor beta (GLRB Products)

Synonyms: HKPX2 antibody, glrb antibody, MGC145156 antibody, AI853901 antibody, Glyrb antibody, spa antibody, spastic antibody, glrb2 antibody, zgc:101041 antibody, glycine receptor beta antibody, glycine receptor, beta antibody, glycine receptor, beta subunit antibody, glycine receptor, beta b antibody, GLRB antibody, Glrb antibody, glrb antibody, glrbb antibody