Synaptojanin 1 antibody (Isoform 2)

Catalog No. ABIN1742316

Product Details anti-Synaptojanin 1 Antibody

Target: Synaptojanin 1 (SYNJ1)

Binding Specificity: AA 1292-1308, Isoform 2

Reactivity: Rat, Mouse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptojanin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Recognizes all isoforms of synaptojanin 1.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide CLPSDSSPQLQQEQP (aa 1292-1308 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 200 up to 1 : 2000 (AP staining)
IP: not tested yet

Comment:

Running SDS-PAGE with non-boiled samples gives stronger signals.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-Synaptojanin 1 antibody (ABIN1742316)

Cliburn, Dunn, Stout, Hoffman, Lohr, Bernstein, Winokur, Burkett, Schmitz, Caudle, Miller: "Immunochemical localization of vesicular monoamine transporter 2 (VMAT2) in mouse brain." in: Journal of chemical neuroanatomy, (2016) (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Park, Kim, Lee, Park, Park, Sun, Kim, Chang: "SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane." in: Journal of cell science, Vol. 123, Issue Pt 10, pp. 1742-50, (2010) (PubMed).

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, (2010) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Shin, Lee, Ahn, Kim, Ahn, YongPark, Chang: "Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis." in: The Journal of biological chemistry, Vol. 282, Issue 39, pp. 28939-50, (2007) (PubMed).

Target Details for Synaptojanin 1

Target: Synaptojanin 1 (SYNJ1)

Alternative Name: Synaptojanin 1 (SYNJ1 Products)

Synonyms: CG6562 antibody, Dmel\\CG6562 antibody, IPP antibody, Synj antibody, fb02f11 antibody, fi15a11 antibody, nrc antibody, wu:fb02f11 antibody, wu:fi15a11 antibody, SYNJ1 antibody, INPP5G antibody, A930006D20Rik antibody, AA675315 antibody, mKIAA0910 antibody, Synaptojanin antibody, synaptojanin 1 antibody, Synj antibody, synj1 antibody, SYNJ1 antibody, Synj1 antibody

Pathways: Inositol Metabolic Process, Synaptic Vesicle Exocytosis