Piccolo antibody (AA 2011-2350)

Catalog No. ABIN1742313

Product Details anti-Piccolo Antibody

Target: Piccolo (PIC)

Binding Specificity: AA 2011-2350

Reactivity: Rat, Mouse

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Piccolo antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for piccolo.

Purification: antiserum

Immunogen: Recombinant rat piccolo (aa 2011-2350).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 200

Comment:

WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. This antibody detects an additional band of ~65 kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Piccolo antibody (ABIN1742313)

Babai, Sendelbeck, Regus-Leidig, Fuchs, Mertins, Reim, Brose, Feigenspan, Brandstätter: "Functional Roles of Complexin3 and Complexin4 at Mouse Photoreceptor Ribbon Synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 25, pp. 6651-67, (2016) (PubMed).

Target Details for Piccolo

Target: Piccolo (PIC)

Alternative Name: Piccolo (PIC Products)

Background: Synonyms: Aczonin