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Bassoon antibody (C-Term)

BSN Reactivity: Rat, Mouse WB, ELISA, ICC Host: Mouse Monoclonal 219E1 unconjugated
Catalog No. ABIN1742310
  • Target See all Bassoon (BSN) Antibodies
    Bassoon (BSN)
    Binding Specificity
    • 13
    • 2
    • 1
    • 1
    C-Term
    Reactivity
    • 25
    • 13
    • 13
    • 1
    • 1
    Rat, Mouse
    Host
    • 25
    • 2
    Mouse
    Clonality
    • 25
    • 2
    Monoclonal
    Conjugate
    • 11
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Bassoon antibody is un-conjugated
    Application
    • 19
    • 18
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    Specificity
    Specific for bassoon.
    Purification
    hybridoma supernatant
    Immunogen
    Recombinant protein containing the 330 C-terminal aa of rat bassoon.
    Clone
    219E1
    Isotype
    IgG2b
    Top Product
    Discover our top product BSN Primary Antibody
  • Application Notes
    WB: 1 : 1000 (AP staining)
    IP: not tested yet
    ICC: 1 : 100 up to 1 : 500
    IHC: not tested yet
    Comment

    ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS
    Handling Advice
    When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
    Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.
    Storage
    -20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).

    Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Schröder, Stellmacher, Romorini, Marini, Montenegro-Venegas, Altrock, Gundelfinger, Fejtova: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).

    Orenbuch, Shalev, Marra, Sinai, Lavy, Kahn, Burden, Staras, Gitler: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).

    Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

    Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).

  • Target
    Bassoon (BSN)
    Alternative Name
    Bassoon (BSN Products)
    Synonyms
    Znf231 antibody, ZNF231 antibody, bassoon antibody, bassoon (presynaptic cytomatrix protein) antibody, bassoon presynaptic cytomatrix protein antibody, Bsn antibody, BSN antibody
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