Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 207-366) antibody

Catalog No. ABIN1742306

Product Details

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Binding Specificity: AA 207-366

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Specific for RIM 1

Cross-Reactivity (Details): no cross reactivity to RIM 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 207-366) of rat RIM 1, a stretch of divergent aa connecting the Rab binding motif and the PDZ domain.

Application Details

Application Notes: WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 500

Comment:

WB: Antibody 3 is recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Target Details

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Alternative Name: RIM 1 (RIMS1 Products)

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity