Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 207-366) antibody

Catalog No. ABIN1742306

Product Details

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Binding Specificity: AA 207-366

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for RIM 1

Cross-Reactivity (Details): no cross reactivity to RIM 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 207-366) of rat RIM 1, a stretch of divergent aa connecting the Rab binding motif and the PDZ domain.

Application Details

Application Notes: WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000
IHC: 1 : 500

Comment:

WB: Antibody 3 is recommended.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Limbach, Laue, Wang, Hu, Thiede, Hultqvist, Kilimann: "Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. E392-401, (2011) (PubMed).

Target Details

Target: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Alternative Name: RIM 1 (RIMS1 Products)

Synonyms: RIMS1 antibody, MGC121671 antibody, C030033M19Rik antibody, RIM1 antibody, RIM1a antibody, RIM1alpha antibody, Rab3ip1 antibody, Rim antibody, Serg1 antibody, mKIAA0340 antibody, CORD7 antibody, RAB3IP2 antibody, RIM antibody, Rim1 antibody, regulating synaptic membrane exocytosis 1 antibody, RIMS1 antibody, rims1 antibody, Rims1 antibody

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity