SDCBP antibody

Catalog No. ABIN1742301

Product Details anti-SDCBP Antibody

Target: SDCBP (Syndecan Binding Protein (Syntenin) (SDCBP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDCBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Detects human protein with higher sensitivity. Recognizes syntenin 1 and 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant full-length human syntenin.

Application Details

Application Notes: WB: 1 : 100 up to 1 : 1000 (AP staining)
IHC: 1 : 1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-SDCBP antibody (ABIN1742301)

Binamé, Sakry, Dimou, Jolivel, Trotter: "NG2 regulates directional migration of oligodendrocyte precursor cells via Rho GTPases and polarity complex proteins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 26, pp. 10858-74, (2013) (PubMed).

Sala-Valdés, Gordón-Alonso, Tejera, Ibáñez, Cabrero, Ursa, Mittelbrunn, Lozano, Sánchez-Madrid, Yáñez-Mó: "Association of syntenin-1 with M-RIP polarizes Rac-1 activation during chemotaxis and immune interactions." in: Journal of cell science, Vol. 125, Issue Pt 5, pp. 1235-46, (2012) (PubMed).

Sulka, Lortat-Jacob, Terreux, Letourneur, Rousselle: "Tyrosine dephosphorylation of the syndecan-1 PDZ binding domain regulates syntenin-1 recruitment." in: The Journal of biological chemistry, Vol. 284, Issue 16, pp. 10659-71, (2009) (PubMed).

Meerschaert, Remue, De Ganck, Staes, Boucherie, Gevaert, Vandekerckhove, Kleiman, Gettemans: "The tandem PDZ protein Syntenin interacts with the aminoacyl tRNA synthetase complex in a lysyl-tRNA synthetase-dependent manner." in: Journal of proteome research, Vol. 7, Issue 11, pp. 4962-73, (2008) (PubMed).

Target Details for SDCBP

Target: SDCBP (Syndecan Binding Protein (Syntenin) (SDCBP))

Alternative Name: Syntenin (SDCBP Products)