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GFP antibody (CypHer5E)

GFP Reactivity: Aequorea victoria ICC Host: Rabbit Polyclonal CypHer5E
Catalog No. ABIN1742297
  • Target See all GFP Antibodies
    GFP (Green Fluorescent Protein (GFP))
    Reactivity
    • 173
    • 15
    • 11
    • 9
    Aequorea victoria
    Host
    • 64
    • 61
    • 34
    • 13
    • 9
    • 5
    • 2
    • 1
    Rabbit
    Clonality
    • 112
    • 72
    Polyclonal
    Conjugate
    • 81
    • 12
    • 11
    • 10
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GFP antibody is conjugated to CypHer5E
    Application
    • 147
    • 80
    • 46
    • 33
    • 26
    • 25
    • 23
    • 21
    • 14
    • 13
    • 13
    • 12
    • 12
    • 5
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunocytochemistry (ICC)
    Cross-Reactivity (Details)
    Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).
    Purification
    Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization.
    Immunogen
    Strep-Tag® fusion protein, full-length GFP.
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    Discover our top product GFP Primary Antibody
  • Application Notes
    IP: not tested yet
    ICC: 1 : 100 up to 1 : 1000
    IHC-P: not tested yet
    Comment

    ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
    Buffer
    PBS
    Handling Advice
    Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
    Storage
    -20 °C
    Storage Comment
    Fluorescence-labeled antibodies should be reconstituted immediately upon delivery.
    Prolonged storage at 4 °C is not recommended!
  • Tatavarty, Sun, Turrigiano: "How to scale down postsynaptic strength." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 32, pp. 13179-89, (2013) (PubMed).

  • Target
    GFP (Green Fluorescent Protein (GFP))
    Alternative Name
    GFP (GFP Products)
    Synonyms
    green fluorescent protein antibody, gfp antibody
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