NLGN1
Reactivity: Rat
WB, IHC, ICC, IF
Host: Mouse
Monoclonal
S97A-31
PerCP
Application Notes
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: not recommended IHC: not recommended
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Dahlhaus, Hines, Eadie, Kannangara, Hines, Brown, Christie, El-Husseini: "Overexpression of the cell adhesion protein neuroligin-1 induces learning deficits and impairs synaptic plasticity by altering the ratio of excitation to inhibition in the hippocampus." in: Hippocampus, Vol. 20, Issue 2, pp. 305-22, (2010) (PubMed).