UNC13A/Munc13-1 antibody (AA 3-317)

Catalog No. ABIN1742277

Product Details anti-UNC13A/Munc13-1 Antibody

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Binding Specificity: AA 3-317

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNC13A/Munc13-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Specificity: Specific for munc 13-1.

Purification: antiserum

Immunogen: Recombinant rat munc 13-1 (aa 3-317).

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200
ELISA: not tested yet

Comment:

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-UNC13A/Munc13-1 antibody (ABIN1742277)

Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

Target Details for UNC13A/Munc13-1

Target: UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))

Alternative Name: Munc 13-1 (UNC13A Products)

Synonyms: Munc13-1 antibody, Unc13h1 antibody, 2410078G03Rik antibody, unc-13 homolog A antibody, unc-13 homolog A (C. elegans) antibody, Unc13a antibody, UNC13A antibody

Pathways: Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis