NSF antibody
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- Target See all NSF Antibodies
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
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Reactivity
- Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NSF antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Specific for NSF.
- Purification
- purified IgG
- Immunogen
- Recombinant full length rat NSF.
- Clone
- 83-11
- Isotype
- IgG1
- Top Product
- Discover our top product NSF Primary Antibody
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- Application Notes
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WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC-P: 1 : 1000 - Comment
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ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742270 as detector antibody (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Storage
- -20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).
: "Preferential increase in the hippocampal synaptic vesicle protein 2A (SV2A) by pentylenetetrazole kindling." in: Biochemical and biophysical research communications, Vol. 390, Issue 3, pp. 415-20, (2009) (PubMed).
: "
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Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).
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- Target
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
- Alternative Name
- NSF (NSF Products)
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